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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been observed in many tumors, which ends up in chromosome instability, and therefore may well lead to tumorigenesis. This protein was uncovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are actually described. Two supplemental splice variants have been reported but specific nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind particularly towards the focus on antibody and block antibody binding. These peptide usually includes the epitope regarded from the antibody.
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This guidebook outlines additional probable optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This guidebook outlines further more probable optimization of mobile and immunoassay parameters to ensure the very best results are...
Antibodies bound to the blocking peptide now not bind to the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody vs . the antibody alone, you can see which staining is particular.
Goal Details Cyclin E belongs for the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells development as a result of S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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